Free energy relationship analysis for temperature dependence of hydride kinetic isotope effects of NADH/NAD + model reactions: implication for barrier compression by enzyme dynamics

Chemical Science 2026 Open access

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Authors: Ava Austin-Kloppe, Nicholas DeGroot, Bikram Dhakal, Jessica Sager, Lauren Phan, Seyedmehrad Poormoghim, Yun Lu
DOI: 10.1039/d6sc01847e
Cited by: 1
References: 86

Abstract

The Λ-shaped free energy relationship for temperature dependence of hydride KIEs, peaking at Δ G ° ∼ 0, is identified in solution, supporting the donor–acceptor distance sampling mechanism and the role of fast protein dynamics in enzyme catalysis.

Fields of Study & Subjects

Biochemical and Molecular Research Chemical Reactions and Isotopes Sirtuins and Resveratrol in Medicine

Publication details

Journal / source
Chemical Science
Publisher
Royal Society of Chemistry (RSC)
Volume
17
Issue
25
Pages
12440–12450
Publication date
1 January 2026
Language
EN