High-dimensional CRISPR-SERS interactomics tracks the topological evolution of plant viral pathogenesis and therapeutic response

Chemical Science 2026 Open access

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Authors: You Dou, Gang Wu, Jun Li, Yuting Wang, Nanke Chen, Tianbing Xu, Xiao-Bo Cai, Tuotuo Zhang, Xinwei Zhang, Xiangyang Li, Junrong Li, Yao Sun
DOI: 10.1039/d6sc03368g
Cited by: 1
References: 47

Abstract

-Potato virus Y (PVY) pathosystem, we dissect the longitudinal evolution of infection under ningnanmycin (NNM) treatment. Beyond enabling presymptomatic diagnosis at Day 1, our interactome analysis reveals that chronic infection stabilizes into a regulatory triangle clamped by the Vpg-PUB4 interface, whereas therapeutic relapse manifests as a chaotic, Vpg-centralized network. This work establishes interactome topology as a superior diagnostic metric to viral load, providing a blueprint for identifying drug resistance mechanisms and targeting the specific protein-protein interactions that drive viral persistence.

Fields of Study & Subjects

CRISPR and Genetic Engineering Plant Virus Research Studies Plant-Microbe Interactions and Immunity

Publication details

Journal / source
Chemical Science
Publisher
Royal Society of Chemistry (RSC)
Volume
17
Issue
28
Pages
13976–13987
Publication date
1 January 2026
Language
EN