Treatment of diabetic wounds using dissolvable microneedle patches co-delivering levofloxacin and lidocaine

, especially for the levofloxacin/lidocaine dual-drug formulation. The pain-relief activity was assessed using a pinch test in diabetic mice, where lidocaine-containing MNPs produced a marked reduction in pain sensitivity compared to blank controls. Furthermore, full-thickness skin wounds in diabetic mice treated with the levofloxacin/lidocaine-loaded MNPs showed accelerated wound-healing over 5 days, as verified by wound-closure measurement and histological H&E staining. The enhanced healing response was attributed to the additive effect of the antibiotic and analgesic agents, resulting in reduced bacterial burden and inflammation, and improved tissue regeneration. These findings suggest that gelatin-based levofloxacin/lidocaine-loaded MNPs offer a promising therapeutic strategy for managing painful skin-wounds under diabetic conditions through localized, sustained, and patient-convenient drug delivery.