Fermentation of Cassava Leaves Using Microbial-Nutrient Additives: Impact on HCN and Fiber Content

This study aimed to evaluate the effectiveness of cassava leaf (Manihot esculenta sp.) fermentation using a combination of microbial (commercial EM4 and local microorganisms from fruit, MOL) and a nutrient additive (commercial Viterna) on the content of hydrocyanic acid (HCN), neutral detergent fiber (NDF), acid detergent fiber (ADF), and pH. The study used four treatments and five replications, namely: T0 (fresh cassava leaf without additives), T1 (fermentation with 40% EM4, 20% ml Viterna, and 40% ml MOL), T2 (20% EM4, 40% Viterna, and 40% MOL), and T3 (40% EM4, 40% Viterna, and 20% ml MOL). Each treatment was applied at a total rate of 0.5% (v/w) fresh cassava leaves. The samples were fermented anaerobically for 14 days. The data were analyzed using analysis of variance based on a completely randomized design and Duncan's further test. The results showed significant differences (P