Development of In vitro Maturation and In vitro Growth Culture Media Using Goat Colostrum

This research aims to develop a more optimal in vitro maturation (IVM) oocyte and in vitro growth (IVG) follicle culture medium through the utilization of goat colostrum as an additive to the standard IVM and IVG culture medium. This research was conducted at the Bioscience Laboratory of Brawijaya University, Malang, using goat ovary samples obtained from the Gadang Slaughterhouse (RPH), Malang Regency. This research was carried out in two stages, namely IVG and IVM, with the data obtained subsequently analyzed using ANOVA followed by LSD for significance testing. IVG was performed on goat follicles with an initial size of 2-3 mm, followed by culture for 6 days using media treated with the addition of goat colostrum from P0 to P4, respectively, 0%, 2.5%, 5%, 7.5%, and 10%. The IVG culture results obtained were observed in terms of follicle size increase, oocyte cell size, and cumulus cell expansion of the oocytes within the follicles. The results obtained were that IVG had no significant effect on follicle size growth (P>0.05), but showed a significant effect on cumulus cell expansion (P