HPLC studies on rosmarinic acid and chlorogenic acid in some Lamiaceae tea plants

Recently, the unstoppable side effects of synthetic drugs have further emphasized medicinal plants as a reliable source of treatment. The Lamiaceae family, one of the most widespread plant families, contains a wide variety of plants that are very active in terms of biological and medicinal applications. This study aimed to determine the amounts of rosmarinic acid and chlorogenic acid, which are valuable phenolic acids, in extracts obtained by aqueous infusion which is used as the herbal tea and extraction with 80% EtOH from 12 Lamiaceae species growing naturally or cultivated in Turkey by High-Performance Liquid Chromatography (HPLC). 12 Lamiaceae species were collected from the Turkish flora [ Salvia rosmarinus ( Rosmarinus officinalis L.), Melissa officinalis L., Origanum onites L., Tymus vulgaris L., Mentha spicata L., Mentha x piperita L., Lavandula x intermedia Emeric ex Loisel., Salvia officinalis L., S. sclarea L., S. triloba L., L. stoechas L. subsp. stoechas , Ocimum basilicum L.]. The chromatographic method used was properly validated according to the procedures specified in ICH guideline Q2(R1) on validation of analytical methods (3). As part of the method validation, validation parameters such as sensitivity, linearity, accuracy, robustness, precision, specificity, and system suitability tests were examined and it was determined that the values obtained were within acceptable limits according to the results of the same-day and different days. According to the results, it was found that the plant extract with the highest rosmarinic acid content was Melissa officinalis L. 80% EtOH extract (3.0 mg/g of dried plant) and the plant extract with the highest chlorogenic acid content was Lavandula x intermedia 80% EtOH extract (0.2 mg/g of dried plant). Phytochemical investigation and screening on biological activity of selected Lamiaceae species are ongoing. These plants are valuable sources of rosmarinic acid and will be promising reservoirs for the future production of pure rosmarinic acid [ 1 ] [ 2 ] [ 3 ]. Funding : this study was supported by the Scientific Research Projects Unit of Hacettepe University (BAP) under Grant Number THD-2024-21621. Publication History Article published online: 05 March 2026 © 2026. Thieme. All rights reserved. Georg Thieme Verlag KG Oswald-Hesse-Straße 50, 70469 Stuttgart, Germany