The impact of Pt and Cu nanoparticles on the production of bioactive compounds and elements – study in in vitro culture model of Brassica oleracea var. acephala (kale)

The study focused on the biotechnological and phytochemical investigation of in vitro cultures of Brassica oleracea L. var. acephala (green kale), a species from the Brassicaceae family. The research aimed to optimize shoot culture conditions and evaluate the effects of supplementing Murashige and Skoog medium (without plant growth regulators) with non-ionic nanoparticles (NPs) of platinum (PtNPs) and copper (CuNPs) at concentrations of 5, 10, and 15 ppm (0 ppm as control). The study assessed the impact of these NPs on polyphenolic compounds, glucosinolate profiles, and elemental bioaccumulation. Polyphenolic analysis (HPLC-DAD) identified protocatechuic, chlorogenic, caffeic, sinapic, ferulic, and rosmarinic acids, as well as flavonoids quercetin and kaempferol, in all extracts. The most significant enhancement of phenolic compound production was observed with 15 ppm PtNPs, leading to a 618% increase in rosmarinic acid content compared to the control. Elemental bioaccumulation analysis (ICP-MS) revealed a concentration-dependent uptake of supplemented NPs, with the highest accumulation (396.45 ppm Pt) detected in shoots treated with 15 ppm PtNPs. Additionally, glucosinolate profiling (UHPLC-HRMS) identified 12 glucosinolates in extracts supplemented with 5 ppm PtNPs, including progoitrin, glucoiberverin, gluconasturtiin, and neoglucobrassicin. These findings highlight the potential of nanoparticle supplementation in modulating bioactive compound production in plant tissue cultures, offering innovative applications for biotechnology and functional food industries. Funding : This research was funded by the National Science Center (Narodowe Centrum Nauki), grant number 2023/49/B/NZ7/02428 Publication History Article published online: 05 March 2026 © 2026. Thieme. All rights reserved. Georg Thieme Verlag KG Oswald-Hesse-Straße 50, 70469 Stuttgart, Germany