KI-67 Immunohistochemical Expression in Lung Tissue of Rats under Chronic Renal Failure
Abstract
Abstract Chronic kidney disease (CKD) is associated with systemic complications, including pulmonary alterations due to kidney–lung crosstalk. This study evaluates Ki-67 immunohistochemical expression in rat lung tissue to assess cellular proliferative activity under experimental chronic renal failure (CRF) conditions, considering age-related variations. Outbred male rats (n = 150, aged 6, 9, and 12 months) were divided into control and experimental groups. CRF was induced via repeated intramuscular injections of 50% glycerol (0.5 mL twice weekly for 1 month). Alveolar lung tissues were fixed, sectioned, and stained for Ki-67 using the 3,3′-Diaminobenzidine method. Quantitative analysis was performed with QuPath software (v0.4.0) to calculate proliferative indices. Data were analyzed using two-way ANOVA with post-hoc tests (p
Fields of Study & Subjects
Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
Chronic Kidney Disease and Diabetes
Cardiac Fibrosis and Remodeling
Publication details
- Journal / source
- Ibnosina Journal of Medicine and Biomedical Sciences
- Publisher
- Georg Thieme Verlag KG
- Volume
- 18
- Issue
- 01
- Pages
- 013–019
- Publication date
- 12 January 2026
- Language
- EN