Biological Effects of Xerosydryles Obtained from Arnica and Belladonna 12cH Homeopathic Dilutions

Background: Homeopathic ultramolecular liquid dilutions exhibit qualities typical of far-from-equilibrium, self-organizing, dissipative systems, unexpected for simple water molecules. They release, after lyophilization, a certain amount of solid material, named Xerosydryle, whose chemical composition is mostly composed of water. When re-dissolved into new water, Xerosydryles transfer the physical-chemical properties of the homeopathic remedies they come from to the new solution. When Xerosydryles from Arnica and Belladonna have been proven in pathogenetic trials, they have shown a remarkable similarity in experimenter symptoms to their origin medicines. These observations have led us to hypothesize that Xerosydryle obtained from those medicines is one of the “active principles” of homeopathic ultramolecular potencies. Objective: To assess the biological properties of Xerosydryles on in vitro systems and to set up an experimental model system to test homeopathic remedies. Methods: Xerosydryles obtained from Arnica and Belladonna 12cH liquid potencies were resuspended in water so that their concentration was 100 times higher than the homeopathic remedy they came. Then they were tested on fibroblasts and Caco-2 cells with a classic MTT assay to assess their cytotoxic activity as well as their anti-inflammatory properties. Results: Xerosydryles from Arnica showed a significant effect on the reduction of inflammation in fibroblasts, both in a preventive and curative way. Belladonna Xerosydryles showed a different behavior, reducing their vitality. Conclusion: Xerosydryles appear to show some biological properties in in vitro systems. These properties seem somehow linked to the properties of the substance of origin (e.g., inflammation and Arnica ). So, they might represent a suitable model system to manipulate and test highly diluted homeopathic medicines for their very subtle biological effects.