RAPD typing of multidrug-resistant Klebsiella pneumonia possessing OqxA and OqxB efflux pump genes

One hundred and thirty clinical samples were collected from Baghdad hospital including urine, sputum, blood, wound and burn infections. Isolation, identification, microscopic examination, biochemical and antimicrobial susceptibility testing were performed. DNA extraction and polymerase chain reaction were then performed to analyses three genes (16S rRNA, oqxA and oqxB). Finally, typing of K. pneumoniae with the RAPD method. The results showed that all K. pneumoniae isolates was 50 (38.4%). The minimum inhibitory concentrations (MICs) test showed that resistance of isolates was 50(100%) to ticarcillin, 43(86%) to ticarcillin/clavulanic acid, 50(100%) to piperacillin, 32(64%) to piperacillin/tazobactam, 39(78%) to ceftazidime, 34(68%) to cefepime, 44(88%) to aztreonam. Genotyping revealed that oqxA was present in 50 (100%) of K. pneumoniae, while oqxB was present in 27 (54%) of isolates. The results of the genotyping of K. pneumoniae using the RAPD method showed that 12 different genotypes with a molecular weight of 250-1500 base pairs.