Study of antimicrobial resistance and biofilm production capability among Klebsiella pneumoniae

Klebsiella pneumoniae is a gram-negative bacillus and is one of the most significant opportunistic bacteria that can cause nosocomial infections or healthcare-associated illnesses. The purpose of this study was to explore the antibiotic resistance of Klebsiella pneumoniae isolates from various chosen hospitals as well as the propensity of these isolates to generate biofilms In all, 74 Klebsiella pneumoniae were isolated from 320 clinical samples that were routinely tested according to the diagnostic laboratory of the selected hospital. These samples included sputum, urine, bloodstream, wound swab, body fluids, pus, tissue swab, endotracheal tube, and ear swab. Antibiotic susceptibility was determined using the agar disc diffusion method for 74 isolates. Resistance rate of cefotaxime n=68 (91.89%), cefepime n=65 (87.84%), imipenem n=49 (66.22%), meropenem n=45 (60.81%), amoxicillin-clavulanic acid n=63 (85.14%), gentamicin n=52 (70.27%), aztreonam n=62 (83.78%), amikacin n=47 (63.51%), and n=40 (54.05%) isolates were classified as multidrug-resistant (MDR). Of these MDR n=28 (70%) were biofilm producers. Increased antibiotic resistance and the biofilm-producing strain have shown serious problems in patients infected with Klebsiella pneumoniae. There is a need to discover an appropriate treatment to eradicate infection with these bacteria to control infection associated with Klebsiella pneumoniae biofilm.