In vitro regeneration of Dendrobium through somatic embryogenesis from leaf explants

The aim of this research was to obtain the best type of basic media and concentration of TDZ for the formation of somatic embryos from Dendrobium orchid leaf explants. This research was carried out using a completely randomized design with 3 replications arranged factorial (2x3). The first factor was the type of basic media, ½ MS and MS. The second factor was the concentration of cytokinin thidiazuron 1, 2 and 3 mg/L. Each experimental unit consisted of 2 culture bottles, each contained 2 explants. The research results showed that 1) The use of both types of basic media was able to induce callus formation on Dendrobium ’Gradita 31’ orchid from leaf explants. 2) The use of 3 mg/L thidiazuron which combined with ½ MS or MS media was able to form primary callus faster than other treatments. 3) The higher percentage for embryo somatic and shoots formation were also found in 3 mg/L thidiazuron.