Estimation study of the optimal conditions for determination of copper (II) spectrophotometrically in an analytical sample

A spectrophotometric approach that is easy to use, quick, sensitive, and precise has been devised to measure copper (II).employing the complexation process with azomethine azo reagent derived from the diazotization reaction between azomethine 4-amino-2-(2-hydroxybenzylideneamino)-1,5-dimethyl-1H-pyrazol-3(2H)-one with 4-nitroaniline create a green-yellow complex when they react with Copper(II) solution at the optimal conditions, pH of reaction equal to 7. The best of reagent concentration is (2×10−4),Sandell’s sensitivity is 9.012×10−4 μg.cm−2 and the complex exhibits λ max at (407 nm) with ɛ of 7.05×104 L.mo1−1.cm−1,Beer’s law has an extraordinarily high degree of linearity in the concentration range of Cu(II) (0.1–12.5 µg.mL−1), as indicated by the R2, value of 0.9994, Whose detection limit is 0.0143 µg.ml−1, and L.O.Q is 0.0474 µg.mL−1, respectively, The complex is nature dictated that the (M:L) ratio was 1:2. This spectrophotometric method is thought to be highly sensitive and accurate. The stability constant of the copper complex was 3.94×108 mol.L−1.