The impact of isolated human Nesfatin-1 from plasma on the histological characteristics of several organs against cisplatin-induced ischemia in rats

Nesfatin-1 is a polypeptide discovered in 2006 in the hypothalamus. it is effect on food intake and energy homeostasis and contributes to modulating the cardiovascular system and inflammatory processes. This study attempted to separate and purify human Nesfatin-1 from plasma of a healthy persons using various biochemical techniques and studying the impact of the separated Nesfatin-1 on the heart and kidneys of albino male rats. Two peaks were isolated from the resulting precipitate using cold acetone and using gel filtration chromatography. Peak B, the second peak, has a high level of Nesfatin-1. Moreover, the purity of the separated Nesfatin-1 (peak B) was determined using gel electrophoresis (GEE) techniques and high-performance liquid chromatography (HPLC). The results of HPLC demonstrated good compatibility in retention time between the separated Nesfatin-1(peak B) and standard solution of Nesfatin-1. The molecular weight of the isolated Nesfatin-1 (peak B) was estimated to be 9200 ± 500 Daltons in using gel filtration chromatography and 10500 ± 100 Daltons in using gel electrophoresis techniques. The impact of isolated nesfatin-1 (peak B) on cisplatin-induced cardiovascular damage in albino male rats was studied and the results showed that nesfatin-1 has therapeutic and protective effects to protect the heart and kidney from cisplatin-induced changes in the typical structure of the heart and kidney through the role of nesfatin-1 in reducing inflammation, oxidative stress and apoptosis.