Adding zeolite-talc mixture during storage of Beauveria bassiana against colony density and chitinase activity

Beauveria bassiana is a pathogenic fungus that is eco-friendly in agriculture. B. bassiana biomass storage is important for maintaining the quality of that fungus. Zeolite and talc are storage media that have some beneficial functions as entomopathogenic bioformulation, such as high molecular binding and improvement of toxicity. The purpose of this research is to examine zeolite and talc as storage media of B. bassiana against colony density and chitinase activity. Formulation of this research contains aquades control treatment,100% zeolite, 100% talc, a mixture of zeolite and talc with concentration ratios: 20%-80%, 40%-60%, 50%-50%, 60%-40%, 80%-20%. Colonies are counted with the Total Plate Count method, and chitinase activity is analyzed with spectrophotometry at 420 nm wavelength. This research consists of eight variables packaged in Completely Randomized Designs with three repetitions, and data will be analyzed with Welch & Brown-Forsythe with Games Howell Multiple Comparison Post Hoc Test. It can be concluded, colony density has significant difference among all of the treatments (Sig.0,000), which Zeolite 100% treatment has the best treatment with 25% decreasing in 28 days. Chitinase activity has significant difference among all treatments (Sig.0,000) which was Zeolite 20%+Talc80% the best formulation with a 35% decrease in 28 days.