Context-dependent variability of HIF heterodimers influences interactions with macromolecular and small molecule partners

Abstract Hypoxia inducible factors (HIFs) are transcription factors that coordinate cellular responses to low oxygen levels, functioning as an α/β heterodimer which binds a short hypoxia response element (HRE) DNA sequence. Prior studies suggest HIF/HRE complexes are augmented by the binding of additional factors nearby, but those interactions are not well understood. Here, we integrated structural and biochemical approaches to investigate several functionally relevant HIF assemblies with other protein, small molecule, and DNA partners. First, we used cryo-electron microscopy (cryo-EM) to establish HIF-1 and HIF-2 self-assemble to form novel “dimer-of-heterodimers” (DoHD) complexes on extended human EPO enhancer sequences, with one heterodimer bound at a canonical HRE site and the second binding in an inverted fashion to an HRE-adjacent sequence (HAS) 8 bp away. Consistent with ARNT PAS-B domains predominating interactions within a DoHD, we found HIF-1 and HIF-2 co-assemble mixed DoHD complexes on the same DNA. Second, we saw that despite the increased complexities of the larger complexes, ligands for the isolated ARNT or HIF-2α PAS-B domains are still capable of binding and disrupting both the heterodimer and DoHD complexes, albeit with variable potencies depending on the ligand. Finally, we combined cryo-EM and hydrogen- deuterium exchange by mass spectrometry (HDX-MS) to show how HIF-1 and HIF-2 heterodimers engage the transforming acidic coiled-coil containing protein 3 (TACC3) coactivator via both ARNT and HIF-α subunits, though this was unseen in the larger DoHD. Our findings highlight the importance of both molecular context and dynamics in biomolecular complex formation, adding to the complexities of potential regulation. Significance Statement Hypoxia inducible factors (HIFs) are transcription factors that regulate oxygen-dependent cellular processes with implications in certain types of cancers. Current molecular structures of HIFs bound to short DNA fragments provide insights into their function, but leave open questions about how they bind longer natural DNA fragments and interact with small molecules and protein coactivators. Integrating structural and biochemical techniques, we discovered a novel assembly in which two HIFs bind together on a single extended DNA fragment, forming a “dimer-of-heterodimers”, which exhibits some differences in ligand and coactivator binding than heterodimers or isolated PAS domains. Our studies highlight how functional contexts can shift structural paradigms and provide greater insight into the mechanisms by which HIFs and similar bHLH-PAS transcription factors operate.