Methionine-specific nonreversible bioconjugation: Advancing precision protein modification
Abstract
We present a methionine-selective, nonreversible bioconjugation strategy that employs activated allylic bromides under mild, aqueous reaction conditions compatible with various peptides and proteins. Compared with conventional allylic bromides, our method improves conjugate stability and suppresses nonspecific reactivity under the examined reaction conditions. This method enables methionine-preferred labeling of peptides and proteins, and provides proof-of-concept applications in covalent inhibitor design and protein functionalization. As a complementary addition to existing methionine bioconjugation strategies, this chemistry expands the toolkit available for protein modification and chemical biology research.
Fields of Study & Subjects
Click Chemistry and Applications
Biochemical and Structural Characterization
Advanced Polymer Synthesis and Characterization
Publication details
- Journal / source
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Volume
- 123
- Issue
- 7
- Pages
- e2519038123–e2519038123
- Publication date
- 10 February 2026
- Language
- EN