Synthesis and cytotoxic evaluation of 2-aminobenzothiazole amide derivatives inspired by hydroxamate structural motifs

In this study, a series of amide derivatives based on the 2-aminobenzothiazole scaffold, structurally inspired by hydroxamic acid (hydroxamate) frameworks, were successfully synthesized in good yields. Their structures were confirmed by spectroscopic techniques, and the compounds were evaluated for cytotoxic activity against MCF-7 and KB human cancer cell lines using the MTT assay. Among the synthesized compounds, compound 3d exhibited notable cytotoxic effects, with IC50 values ranging from 27.06 to 85.28 µM. Preliminary structure–activity observations suggest that modifications of the amide functionality and the benzothiazole ring influence the observed cytotoxicity. These findings provide preliminary insights into the cytotoxic potential of benzothiazole-based amide derivatives and support further mechanistic investigations.