Molecular Characterization, Antimicrobial, and Siderophore Activity of Myxobacteria Isolated from Selected Soil Samples

Myxobacteria are thought of as “microbe factories” for active secondary metabolites; there is a great desire to discover various medical uses for myxobacteria from different settings. Myxobacteria release lytic enzymes that hydrolyze macromolecules and lyse the target cells. In this study, Myxobacteria were compared with other bacteria in terms of producing enzymatically active lytic molecules, fruiting bodies, and siderophore formation with antibacterial and antifungal potential. Soil samples were collected from different regions of Mansehra. A microscopic and biochemical examination was performed for the confirmation of isolates; antimicrobial activity was observed against Klebsiella pneumoniae, Staphylococcus aureus, Escherichia coli, Enterobacter cloacae, Pseudomonas aeruginosa, and Aspergillus flavus, lytic molecules were also produced by isolated strains of Myxobacteria, and molecular identification was performed using 16S rRNA. These discoveries broaden our knowledge of the diversification of Myxobacteria, and the methods discussed here will be helpful for upcoming studies on the physiological characteristics of these intriguing and important commercial entities. These findings add to our understanding of the biodiversity of Myxobacteria, as well as the approaches presented here will be beneficial for future research on the physiology of these fascinating and commercially significant creatures.