Ozonated Sunflower Oil as a Treatment for Full-Thickness Burns Induced by CO 2 Laser in Rat Skin: Histometric Analysis

This study aimed to investigate the influence of topical ozonated sunflower oil (OSO) on CO2 laser-induced skin burns in rats through quantitative histometric analysis of fibroblasts, mononuclear inflammatory cells, and blood vessels. Thirty healthy adult rats were divided into two groups of 15 animals each: OSO group and Control groups. Histological fields were quantitatively assessed at three time intervals. Quantitative histometric analysis demonstrated that topical OSO significantly modulated the wound healing process. Compared to the Control group, OSO-treated wounds maintained higher fibroblast and vascular densities at 14 and 21 days. Additionally, statistically significant temporal changes in mononuclear inflammatory cells and blood vessels were evidenced within each group. By day 21, the OSO group showed a statistically significant increase in fibroblast density (p = 0.002), mononuclear inflammatory cell presence (p = 0.015), and vascular density (p = 0.046), indicating accelerated and enhanced tissue repair dynamics. In conclusion, the topical OSO application effectively modulated the reparative response in CO2-induced skin burns in rats, with a positive impact on the densities of fibroblasts, mononuclear inflammatory cells, and blood vessels. These findings underscore the clinical promise of OSO as an adjunctive therapy for skin burns, promoting a regenerative microenvironment conducive to optimal wound healing.