Optimization of agarose–gelatin double embedding to minimize sectioning artifacts in multicellular spheroids

BioTechniques 2026 Open access

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Authors: Hyoyoung Maeng, Min-Gi Han, Yoseop Jeon, Donghyeon Kim, Yuna Park, Hyuk Song
DOI: 10.1080/07366205.2026.2621053
Cited by: 0
References: 14

Abstract

Processing methods that do not induce shape distortion are essential for the analysis of tissue morphology. Although the use of agarose-gelatin double embedding to reduce shape distortion has been suggested, the effect of matrix concentration has not been addressed. Therefore, combinations of agarose (1-3%) and gelatin (1-10%) were evaluated using multicellular spheroids without extracellular matrix as a model of mechanical fragility. In this study, a blend of 2% agarose and 5% gelatin effectively prevented distortion. This protocol can enhance the morphological analysis of delicate tissues.

Fields of Study & Subjects

Medical and Biological Sciences 3D Printing in Biomedical Research Hydrogels: synthesis properties applications

Publication details

Journal / source
BioTechniques
Volume
78
Issue
1-12
Pages
1–5
Publication date
2 February 2026
Language
EN