Stability indicating RP-HPLC method for the simultaneous determination of multi-flavonoid compounds in herbal extracts, besides green analytics

Natural Product Research 2026

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Authors: Vaishnavi Ramakant Dhampalwar, Naresh Pothuraju, Ramalingam Peraman
DOI: 10.1080/14786419.2026.2613281
Cited by: 2
References: 5

Abstract

A stability-indicating RP-HPLC method was developed and validated for the simultaneous quantification of five flavonoids, viz., epigallocatechin gallate (EGCG), silibinin (SL), naringenin (NG), genistein (GT), and apigenin (AG), in herbal extracts. Chromatographic separation was achieved on a C18 column using 0.1 M ammonium acetate and methanol at 40:60% v/v. The method demonstrated acceptable resolution, linearity (r2: 0.9905–0.9969), precision (98%. The method demonstrated acceptable green chemistry by the AGREES and GAPI tools.

Fields of Study & Subjects

Silymarin and Mushroom Poisoning Tea Polyphenols and Effects Flavonoids in Medical Research

Publication details

Journal / source
Natural Product Research
Publisher
Informa UK Limited
Pages
1–6
Publication date
13 January 2026
Language
EN