Lanostane triterpenoids from the fruiting bodies of Ganoderma multipileum and their anti-inflammatory activities

A previously unreported lanostane-type triterpenoid designated as (25S)-3β,15β-dihydroxy-7,11,23-trioxo-lanost-8,17(20)-dien-26-oic acid (1) and fifteen known compounds (2–16), was obtained from Ganoderma multipileum fruiting bodies. Combined analysis of NMR and HRESIMS data, together with theoretical computations involving NMR chemical shift prediction and DP4+ analysis, the structural elucidation of the new compound was accomplished. Bioactivity screen was performed on all compounds to assess their anti-inflammatory activity. Notably, in LPS-stimulated RAW264.7 cells, compounds 4 and 5 inhibited the NO generation, yielding IC50 values of 12.89 ± 0.50 and 27.55 ± 2.95 μM, respectively, with potency close to that of quercetin (IC50: 11.80 ± 1.75 μM).