Nitrogen blowing DµSPE of sunitinib, imatinib, and dasatinib using LDH/MIL-101(Cr)-NO 2 composite prior to HPLC-PDA

Background The therapeutic bioanalytical challenge activity of tyrosine kinase inhibitors (TKIs) can be assessed by measuring them in biological samples such as plasma and serum. Determining these compounds requires an efficient sample before the analysis to minimize matrix effects and enhance preconcentration. This study presents the use of a composite of layered double hydroxide (LDH) and metal organic framework (MOF) composite for extracting and preconcentrating of sunitinib, imatinib, and dasatinib.Methodology The proposed composite was synthesized through the in-situ growth of MIL-101(Cr)-NO2 MOF on the MgFe-LDH surface. The extraction process involves adding 5 mg of LDH/MIL-101(Cr)-NO2 to the plasma solution containing the analytes, under nitrogen blowing. Centrifugation separated the analyte-loaded sorbent, which was then eluted with 125 μL of mobile phase. The organic phase was used subsequent high performance liquid chromatography (HPLC) – photo diode array detector (PDA) analysis.Results Under optimum conditions, the developed method demonstrates favorable extraction recoveries (66–74%), low limits of detection (LOD) and quantification (LOQ) (0.46–0.69 ng mL−1 and 1.5–2.2 ng mL−1, respectively), wide linear ranges (LRs) (1.5–200 ng mL−1) with high coefficients of determination (0.997–0.999) and proper repeatability (RSD ≤ 6.6%).Conclusions Notably, the method provides the use of minimal amounts of sorbent and solvents, short extraction time, no need for complicated instruments, and high performance in monitoring anti-cancer drugs in biological samples.