Development and validation of a sensitive HPLC-MS/MS method for the analysis of semaglutide in human plasma
Abstract
BACKGROUND: Semaglutide is a human glucagon-like peptide-1 (GLP-1) peptide analog. To date, the literature does not report a validated HPLC-MS/MS assay using a triple-quadrupole mass spectrometer for the quantitation of semaglutide in human plasma in support of pharmacokinetics. A robust, sensitive HPLC--MS/MS assay supporting this class of compounds, is provided and full validation demonstrated. METHODS AND RESULTS: A triple-quadrupole HPLC-MS/MS method has been developed and fully validated for the quantitation of semaglutide in human plasma, using a stable isotope labeled internal standard and an effective, efficient protein precipitation sample extraction. The method has a sensitive analytical range of 5 ng/mL to 200 ng/mL (and to 1000 ng/mL with a dilution QC) and exhibits excellent accuracy (92.7% to 109.4%) and precision of
Fields of Study & Subjects
Diabetes Treatment and Management
Metabolism
Diabetes
and Cancer
Hyperglycemia and glycemic control in critically ill and hospitalized patients
Publication details
- Journal / source
- Bioanalysis
- Publisher
- Informa UK Limited
- Volume
- 18
- Issue
- 11
- Pages
- 971–980
- Publication date
- 24 July 2026
- Language
- EN