Bioprinting collagenase-responsive hydrogel for controlled release of cowpea mosaic virus immunotherapy

Biofabrication 2026 Open access

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Authors: Zhongchao Zhao, Yi Xiang, Zhuohong Wu, Yazhi Sun, Jacob Schimelman, Steven Fiering, Shaochen Chen, Nicole Steinmetz
DOI: 10.1088/1758-5090/ae37de
Cited by: 3
References: 36

Abstract

In this work, we developed a collagenase-responsive hydrogel system to covalently load cancer immunotherapy candidate cowpea mosaic virus (CPMV) using 3D digital light processing bioprinting technology. CPMV was functionalized with norbornene groups (CPMV-NB), which was then bioprinted into hydrogels with 8-arm polyethylene glycol norbornene and a collagenase-cleavable peptide via photoinduced thiol-ene click chemistry. This strategy enabled stable retention of CPMV-NB within the hydrogels and achieved controlled release of CPMV-NB triggered by collagenase. Furthermore, released CPMV-NB retained its immunogenicity to stimulate immune cells.

Fields of Study & Subjects

3D Printing in Biomedical Research Advanced Biosensing Techniques and Applications Biosensors and Analytical Detection

Publication details

Journal / source
Biofabrication
Publisher
IOP Publishing
Volume
18
Issue
1
Pages
015027–015027
Publication date
13 January 2026
Language
EN