A comprehensive database for biological data derived from sewage in five European cities

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Authors: Ágnes Becsei, Patrick Munk, Alessandro Fuschi, Saria Otani, József Stéger, Dávid Visontai, Krisztián Papp, Christian Brinch, Ravi Kant, Ilya Weinstein, Olli P. Vapalahti, Miranda de Graaf
+23 more Claudia M. E. Schapendonk, Jeroen H. Roelfsema, Maaike J. C. van den Beld, Roan Pijnacker, Eelco Franz, Patricia Alba, Antonio Battisti, Alessandra De Cesare, Valentina Indio, Fulvia Troja, T. Sironen, Chiara Oliveri, Frédérique Pasquali, Ivan Liachko, Benjamin Auch, Colman O’Cathail, Krisztián Bànyai, Magdolna Makó, Péter Pollner, Marion Koopmans, Istvan Csabai, Daniel Remondini, Frank M. Aarestrup
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References: 32

Abstract

Sewage metagenomics is a powerful tool for proactive pathogen surveillance and understanding microbial community dynamics. To support such efforts, we present a highly curated and accessible longitudinal dataset of 239 sewage samples collected from five European cities. The dataset, processed through metagenomic sequencing, includes rich analytical outputs such as taxonomic profiles, identified antimicrobial resistance genes, assembled contigs with annotated origins, metagenome-assembled genomes with functional gene annotations, and metadata. Given the computational intensity and time required to reproduce such analyses, we share this dataset to promote reuse and advance research. In addition to the metagenomic data, qPCR was used to identify specific pathogens, and Hi-C sequencing was performed on a subset of the samples to strengthen genomic linkage analysis. Central to this resource is a publicly available PostgreSQL database, designed to facilitate efficient exploration and reuse of the data. This comprehensive database allows users to perform targeted queries, subset data, and streamline access to this extensive resource.

Fields of Study & Subjects

Genomics and Phylogenetic Studies Pharmaceutical and Antibiotic Environmental Impacts Bacteriophages and microbial interactions

Publication details

Journal / source
Database
Volume
2026
Publication date
1 January 2026
Language
EN