L26/P-072 Efficacy of sperm selection device for ICSI: a sibling-oocyte study of blastocyst quality and euploidy

Abstract Study question What is the impact of using a sperm selection device (SSD) on blastocyst quality and euploidy when compared to a conventional sperm preparation method? Summary answer Interim analysis shows that SSD yields blastocyst quality and euploidy rates comparable to density gradient preparation (DGP). What is known already Obtaining functionally competent sperm cells is the first step in infertility treatment. New trends in sperm selection methods incorporate microfluidic devices using microchannels and membranes to isolate motile sperm. Unlike traditional DGP, microfluidic platforms may exploit additional properties such as rheotaxis, chemotaxis, and thermotaxis, while avoiding centrifugation-related stress. As a result, SSD has been proposed to enrich sperm with lower DNA damage as centrifugation is avoided. However, evidence remains limited and inconsistent, and further controlled studies are required to validate the clinical effectiveness of these devices. Study design, size, duration This study presents an interim analysis of an ongoing prospective randomized trial performed in a single tertiary centre between September and December 2025. The analyses included 388 mature oocytes (MII) from 40 patients inseminated using a sibling-oocyte design with sperm prepared by either DGP (PureCeption™, 45%-90%) or SSD (Zymot™). Blastocysts graded ≥BL3CC (Gardner) on Day (D) 5-7 were biopsied for PGT-A. Associations between sperm preparation methods, blastocyst quality, euploidy rates, and morphokinetic parameters were evaluated. Participants/materials, setting, methods Included patients had a female age