Determination of Per- and Polyfluoroalkyl Substances in Food and Feed by Enhanced Matrix Removal LC-MS/MS Method, AOAC Official Method 2025.11, First Action

BACKGROUND: AOAC INTERNATIONAL Standard Method Performance Requirement (SMPR)® 2023.003 outlines method performance requirements for the determination of per- and polyfluoroalkyl substances (PFAS) across a broad range of food and feed matrixes. OBJECTIVE: The objective of this study was to develop and validate the EMR LC-MS/MS method in accordance with AOAC INTERNATIONAL SMPR 2023.003 using a single-laboratory approach. METHODS: The method features QuEChERS extraction followed by a novel enhanced matrix removal (EMR) mixed-mode passthrough cleanup, an online injection program, sensitive LC-MS/MS detection, and accurate, precise quantitation using neat calibration curves with isotopically labeled internal standards. RESULTS: Method performance was evaluated for suitability and selectivity, calibration linearity, limit of quantitation (LOQ), recovery, and repeatability, and the method was successfully validated in a single-laboratory validation (SLV) across 16 diverse food matrixes, encompassing all 11 required food categories. Acceptable PFAS background cleanliness was demonstrated, supporting accurate and reliable quantitation in all food matrixes. Validated or calculated LOQs for all analytes across all food matrixes were equal or below the required LOQs, with an exception rate of