Micro-QuEChERS extraction method optimization for quantification of designer benzodiazepines in forensic postmortem blood samples

Journal of Analytical Toxicology 2026

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Authors: Karla Aparecida de Oliveira Souza, Janaína Aparecida Reis Teodoro, Débora Zorrón Berlinck, Ana Paula Knak Rezendes, Daniela Mendes Louzada de Paula, Fabrício Souza Pelição, Elvis Medeiros de Aquino, Victor Alexandre Percínio Gianvecchio, Maurı́cio Yonamine, José Luiz Costa
DOI: 10.1093/jat/bkag008
Cited by: 1
References: 47

Abstract

The aim of this study was to develop and validate a quantitative method for the analysis of designer benzodiazepines in postmortem blood samples using micro-QuEChERS extraction and liquid chromatography tandem mass spectrometry (LC-MS/MS). A comprehensive optimization of the method was performed using a multivariate statistical approach, incorporating validation criteria in line with established practices for method validation in forensic toxicology. The method showed linearity between 1 and 200 ng/mL (r2 > 0.990), with good imprecision (

Fields of Study & Subjects

Forensic Toxicology and Drug Analysis Carcinogens and Genotoxicity Assessment Psychedelics and Drug Studies

Publication details

Journal / source
Journal of Analytical Toxicology
Publisher
Oxford University Press (OUP)
Volume
50
Issue
4
Publication date
3 February 2026
Language
EN