Genomic description of the first multidrug-resistant Nakaseomyces glabratus ( Candida glabrata ) strain detected in Costa Rica

This report describes the first discovery in Costa Rica of a multidrug-resistant strain of Nakaseomyces glabratus (formerly Candida glabrata). The infection occurred in a 39-year-old man who had a complicated medical history, with repeated intra-abdominal infections, and eventually died from septic shock. Antifungal susceptibility testing was performed, and Illumina whole-genome sequencing was conducted to identify mutations associated with the resistance profile. Genomic analysis identified critical non-synonymous mutations in key resistance genes, linked to azole and echinocandin resistance. This case underscores the critical threat of antifungal resistance and highlights the importance of genomic surveillance in understanding resistance mechanisms to guide public health responses.