CALCIUM-DEPENDENT PROTEIN KINASE9 negatively regulates cold tolerance by phosphorylating the NADPH oxidase VpRBOHD in grapevine

PLANT PHYSIOLOGY 2026

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Authors: Yu Zhang, Haoke Wang, Xuanyang Zan, Ruixin Tang, Arij Khalil, Q. W. Zhang, Jiale Wen, Jianyan Guo, Zijian Liu, Heqiong Wang, Yipeng Zhang, Chengjie Hou
+5 more Tao Li, Chundong Niu, Ye Guo, Yingqiang Wen, Qingmei Guan
DOI: 10.1093/plphys/kiag170
Cited by: 1
References: 49

Abstract

Calcium-dependent protein kinases (CPKs) are key regulators of plant responses to abiotic stress; however, their roles in grapevine under cold stress remain poorly understood. In this study, we demonstrate that cold-repressed VpCPK9 and its counterpart NADPH oxidase VpRBOHD negatively regulate cold tolerance in grapevine. VpCPK9 interacts with VpRBOHD and phosphorylates VpRBOHD at Ser33, Ser132, and Ser138. This phosphorylation enhances VpRBOHD stability, thereby promoting reactive oxygen species (ROS) production under cold conditions, leading to reduced cold tolerance of grapevine. Our study reveals a negative role of the VpCPK9-VpRBOHD module in grapevine cold response and provides candidate genes for improving cold tolerance through genome editing.

Fields of Study & Subjects

Plant Stress Responses and Tolerance Plant responses to water stress Plant Gene Expression Analysis

Publication details

Journal / source
PLANT PHYSIOLOGY
Publisher
Oxford University Press (OUP)
Volume
201
Issue
2
Publication date
26 March 2026
Language
EN