Performance, thermoregulation, and liver function in beef heifers exposed to endophyte-infected or endophyte-free tall fescue under a common environment

Abstract Fescue toxicosis, induced by ingestion of ergot alkaloids from endophyte-infected tall fescue, remains a significant challenge to beef cattle production. This study evaluated the effects of fescue toxicosis on performance, thermoregulation, and liver metabolism in beef heifers maintained under the same experimental conditions as non-exposed controls. Twenty-four commercial Angus heifers were randomly assigned to a diet containing either an endophyte-infected (E+) or endophyte-free (E−) seeds for 49 days. Heifers were allocated to balance genetic representation across the two dietary groups to minimize genetic confounding. Environmental data, including temperature and relative humidity, were continuously recorded, and minimum, mean, and maximum temperature-humidity index (THI) were calculated each day. Two heatwave events occurred during the trial. E + heifers exhibited reductions in dry matter intake (DMI), feeding rate, meal size, average daily gain (ADG), and body weight, and elevated respiration rate and rectal temperature compared to E− heifers (P