Correction to “Endothelial Cells From Umbilical Cord of Women Affected by Gestational Diabetes: A Suitable In Vitro Model to Study Mechanisms of Early Vascular Senescence in Diabetes”

P. Di Tomo, N. Alessio, S. Falone, et al., “Endothelial Cells From Umbilical Cord of Women Affected by Gestational Diabetes: A Suitable In Vitro Model to Study Mechanisms of Early Vascular Senescence in Diabetes,” FASEB Journal 35 (2021): e21662, https://doi.org/10.1096/fj.202002072RR. The authors inadvertently reused and relabeled the RB panel in Figure 5C as the p16 panel in Figure 5C with a horizontal flip. The RB image has been replaced with the corrected image taken contemporaneously with the other blot images. This error does not affect the interpretation of the results or the conclusions of the study. Additionally, the same β-actin loading control was used for Figure 1B with SOD1 as the experiment as in Figure 3B with SOD2 as the experiment, as both experiments were performed simultaneously. This reuse was not declared in the original text. The authors apologize for the errors. FIGURE 5. GD-HUVECs exhibit senescence phenotype, as compared to C-HUVECs. In (A) the scatter plot shows the percentage (%) of β-galactosidase positive cells; the representative images indicate the positive cells (blue cells) for acid β-galactosidase staining (400×). Data are expressed as mean ± SD. Both C- and GD-HUVECs, n = 3. Student's t-test: **p < 0.01 versus C-HUVECs. (B) The graph shows the degree of H2AX phosphorylation and each dot represents one cell. A representative microscopic field is shown (400×). The foci number was determined for 200 cells. (C) The picture shows western blot analysis detection for the most protein involved in P16-RB and P21-P53 pathways. The histogram shows mean ± SD expression protein levels. Both C- and GD-HUVECs, n = 5. Histograms in (D) and (E) show the proliferation level in C- and GD-HUVECs culture evaluated at 48 h by MTT assay and cell number, respectively. Data are expressed as mean ± SD. Both C- and GD-HUVECs, n = 10 (D) and n = 6 (E). Student's t-test: *p ≤ 0.05 versus C-HUVECs.