Targeting Oral Tissue Regeneration With Standardized Centella asiatica Extract: Experimental Insights From Human Fibroblast Cultures

OBJECTIVES: The possible cytotoxic effect of Centella asiatica extract on human dermal fibroblast cells was examined in this cell culture experiment. The main objective was to determine its basic safety for potential use in oral tissue regeneration applications and to assess its biocompatibility in vitro. METHODS: Dulbecco's Modified Eagle Medium with fetal bovine serum was used to cultivate human dermal fibroblasts. Using the conventional MTT experiment, cell viability was quantitatively evaluated. At concentrations of 5, 10, 50, 100, 150, and 200 g/mL, Centella asiatica extract was applied to cell cultures. A positive cytotoxicity control was Triton X-100, while untreated cells were used as the negative control. To determine dose-response associations (P