Salinimicrobium aquimaris sp. nov., a novel Flavobacteriaceae member isolated from seawater in the tidal region of Danzhou, Hainan Island

A yellow, Gram-stain-negative, facultatively anaerobic and rod-shaped strain, designated as HB62 T , was isolated from seawater in the tidal region of Danzhou, Hainan Island, People’s Republic of China. Growth of strain HB62 T occurs at 16–40 °C, 0.5–10.0% (w/v) and pH 6.0–9.5 with the optima of 37 °C, 1.5–2.0% (w/v) and pH 6.5–7.0. Strain HB62 T could grow anaerobically by using nitrate and sulphate as electron acceptors. The 16S rRNA gene sequence identity analysis showed that strain HB62 T shared the highest sequence identity of 98.0% with Salinimicrobium soli CAU 1287 T and those of ≤97.9% and ≤94.6% with the rest of Salinimicrobium type strains and other genera type strains, respectively. Phylogenetic trees based on 16S rRNA genes and single-copy orthologous clusters revealed that strain HB62 T was stably clustered into the genus Salinimicrobium . Average nucleotide identity and digital DNA–DNA hybridization values of strain HB62 T and Salinimicrobium type strains were 72.1–78.2% and 17.8–21.2%, respectively, which were lower than the species delineation thresholds. The sole respiratory quinone was identified as menaquinone-6. Major fatty acids (>10%) are iso-C 15:0 , iso-C 16:0 and summed feature 9 (iso-C 17:1 ω 9c and/or C 16:0 10-methyl). Polar lipids included phosphatidylethanolamine, two unidentified glycolipids, three unidentified lipids and two unidentified aminolipids. Based on the genetic, chemotaxonomic and phenotypic results, strain HB62 T is concluded to represent a novel Salinimicrobium species, for which the name Salinimicrobium aquimaris sp. nov. is proposed. The type strain is HB62 T (=KCTC 102226 T =MCCC 1K08856 T ).