Description of Pseudomonas stagnisoli sp. nov. and Pseudomonas cimarronensis sp. nov., isolated from freshwater sediments

A polyphasic taxonomic approach was employed to characterize two novel strains, P3C3 T and MAC6 T , isolated from sediments of the Innovation Way Drive Pond (Stillwater, OK) and the Cimarron River (Perkins, OK), respectively. Both strains were strictly aerobic, Gram-stain-negative, rod-shaped, motile and non-spore-forming bacteria. Strain P3C3 T grew optimally at 35 °C, pH 6.0 and 0.5% (v/w) NaCl, while strain MAC6 T grew optimally at 30 °C, pH 6.0 and 4.0% (v/w) NaCl. Based on 16S rRNA gene sequence similarity, P3C3 T was most closely related to Pseudomonas alcaligenes (98.3%), whereas MAC6 T was most closely related to Pseudomonas anguilliseptica (98.6%). Phylogenetic analysis using the 16S rRNA gene and whole-genome phylogenomic analysis indicated that P3C3 T and MAC6 T formed distinct branches within the Pseudomonas genus. Additionally, overall genomic-based relatedness indices demonstrated that P3C3 T and MAC6 T and the type strains of close relatives shared average nucleotide identity and digital DNA–DNA hybridization values