Thermally Robust Cooperative Function in Mechanical Networks

Elastic networks can be tuned to exhibit complex mechanical responses and have been extensively used to study protein allostery, where a localized strain regulates the conformation at a distant site. We show that cooperative binding, where one site enhances the other's ability to function, can be improved by tuning for bidirectional allostery: a symmetric coupling where the strain propagated between the two sites is independent of which site is perturbed. We identify a crossover temperature above which functionality breaks down due to thermal fluctuations. We introduce a modified tuning process to increase this crossover temperature, showing that function can be robust at biologically relevant temperatures if thermal stability is important.