Multiphoton Imaging With Frequency Encoded Illumination Using Dual Acousto-Optic Deflectors
Abstract
We report on the prototype and design of a new multimodal acousto-optic deflector based multiphoton/structured light microscope. The prototype features two orthogonally arranged acousto-optic deflectors (AODs) integrated into a Mach- Zehnder interferometer, enabling fast, flexible light pattern generation. Two operational modalities are supported: camerabased detection and single-pixel acquisition using an avalanche photodiode (APD) with frequency-encoded illumination. We describe the optical design, alignment procedure, and performance of both modalities, supported by experimental data from structured illumination and frequency-domain image reconstruction
Fields of Study & Subjects
Optical and Acousto-Optic Technologies
Advanced Fluorescence Microscopy Techniques
Photoacoustic and Ultrasonic Imaging
Publication details
- Journal / source
- IEEE photonics journal
- Volume
- 18
- Issue
- 2
- Pages
- 1–6
- Publication date
- 2 February 2026
- Language
- EN