Investigation of Electrocatalysis for Electrical Field-Based Anti-Interference Histamine Biosensor With Readout Circuit

This work introduces an electrical field based biosensor for accurate quantification of histamine, featuring a polyvinyl chloride (PVC) membrane. The device employs a highly sensitive analog front-end circuit with an 8 μV low-drift voltage offset, enabling detection over a wide concentration range of 0.02 μM to 100 μM, achieving a sensitivity of 12.35 mV/μM. When exposed to histamine in the presence of diamine oxidase (DAO), the sensor exhibited a pronounced signal change of 63%. Marked signal suppression occurs exclusively under severe interference conditions, specifically when the concentration ratio of histamine to putrescine, cadaverine, and tyramine reaches 1:20. Owing to its excellent sensitivity and selectivity, the proposed histamine biosensor is well suited for rapid, on-site histamine detection in food and fish markets, offering strong potential for food safety monitoring applications.