Pipette-Free Operation of Biomedical Microdevice for Field-Deployable Molecular Diagnostics

A molecular diagnostic microdevice integrated with DNA extraction, loop-mediated isothermal amplification (LAMP), and colorimetric detection functionalities was developed for a rapid on-site identification of pathogens in a pipette-free manner. The device enables LAMP operation at 40 °C and the DNA extraction and solid-phased based colorimetric detection were performed at room temperature in a continuous manner using a PMMA microdevice. The microdevice demonstrated a technical innovation for constructing a portable biomedical microdevice for fielddeployable diagnostics in a power-free manner.