Pneumatic Valve-Integrated Magnetic Levitation-Based Microfluidic System for in-Situ Medium Exchange

In this study, a pneumatic valve-controlled microfluidic magnetic levitation (MagLev) platform was developed that enables rapid, on-chip media exchange without altering the levitation height of microparticles. For this purpose, the platform was designed with a threechamber structure separated by valves that allow for independent media control and flow change. Microparticles with a density of$1.035 \mathrm{g} / \text{mL}$were able to withstand varying flow rates in media containing 50-100 mM gadolinium without microparticle loss, and the chamber media could be exchanged within minutes. This study offers an innovative approach to studies requiring dynamic density measurement and opens new opportunities for automated, real-time separation and analysis applications on MagLev platforms.