From Dlp Printing to Dle Integration: Streamlined Fabrication of Cell Monitoring Platforms

This work introduces a rapid print-pause-print digital light processing (DLP) method that integrates porous membranes and flexible electronic layers directly into a single photo-curable resin structure through digital light encapsulation (DLE). This straightforward process eliminates conventional molding, bonding, and extracellular matrix (ECM) coating steps, enabling device fabrication within minutes. The resulting platform supports epithelial cell adhesion and monolayer formation without ECM pretreatment. Embedded capacitive electrodes allow real-time monitoring, with capacitance changes strongly correlating with actual cell morphology. This rapid and scalable approach provides an efficient method for constructing multimaterial microdevices for monitoring cell culturing process.