Mechano-Index Activated Sorting by Utilizing Acoustic Signal in a Multi-Layered Microfluidic Chip Made by High-Rigid Polymer

We present a high throughput sorting system activated by acoustic impedance characterized using non-contact measurement method. Previous characterization methods using contact probes offer high resolution but suffer from low throughput. Therefore, we constructed a sorting system based on non-contact mechanical characterization, which consists of 3D flow focuser, ultrasound microscope, and high-speed on-chip sorting system. Since the entire process is performed under fast flow conditions, the system has capability of mechano-index activated sorting with highthroughput. To integrate these technologies, we employed the multi-layered microfluidic chip fabricated by utilizing femtosecond laser processing of high-rigid polymer films. As a results, we successfully demonstrated the on-chip mechano-index activated sorting using$100-\mu \mathrm{m}$polystyrene microbeads and achieved high-throughput particle sorting of 67 Hz.