Microlfuidic Device for Bovine Sperm Sex Sorting

Sex sorting of bovine sperm is a critical technique in the livestock industry to predetermine the sex of offspring, thereby enhancing herd management, breeding efficiency and economic value. Commercial methods such as flow cytometry, though effective, are expensive, complex, and may compromise sperm viability and health due to highpressure and staining processes. The proposed novel bionic microfluidic platform utilizes silica-coated magnetic nanoparticles with selective binding affinity to Y chromosome-bearing sperm cells. By embedding a magnetic strip underneath the microfluidic channel, Ysperm cells are efficiently trapped, while X-sperm cells continue unimpeded toward collection with an efficiency of 70% in X and$80 \% \mathrm{Y}$.