Slidable Lateral Flow 2D Chromatography Cassette with Through-Hole Electrospray Ionization and Desorption for High Performance MS Analysis

This paper describes a new 2D chromatography cassette with through-hole electrospray ionization and desorption for high performance MS analysis. The paper chip is produced by high precision IR laser for two-dimensional paper chromatography. The innovative design of the paper chip can boost the effectiveness of 2D chromatography and simultaneously increase the ion intensity. This study systematically studied the ion intensity which affected by 1st and 2nd dimensional chromatography performance under different distance between MS inlet and ESI tip. In addition, this study also discussed the effectiveness of on-chip thermal plate desorption. The results perform the capability of 2D Chromatography Cassette for detecting the bio-sample and the mixed sample. Successfully detecting different species in the Chinese herb medicines and the extracted solution of camphor leaves. The developed slidable lateral flow cassette has shown its potential in the application of complex samples.