A Microfluidic Picoinjection Platform for DNA Fragment Assembly

This study presents a novel microfluidic picoinjection[1] platform designed to achieve efficient, precise, and automated DNA assembly. The platform integrated as many as 10 droplet picoinjectors preloaded with different DNA fragments based on the Overlap Extension PCR(OE-PCR) principle and successfully assembled DNA chains up to 10 fragments in one pot. The presented system allows flexible selection of DNA fragments from any picoinjector to synthesize DNA constructs of varying lengths and types. It offers operation simplicity and low system cost compared to existing microfluidic DNA assembly methods [2]–[4] that requires digital microfluidics, complex valve control, or metafluidics.