An Ultrasensitive Electrochemiluminescent Microfluidic Sensor for Dual Detection of Waterborne Foodborne Pathogens

This study reported the development of an electrochemiluminescence immunoassay (ECLIA) immunosensor integrating microelectrodes with a microfluidic system, which, when combined with a selfdeveloped ECLIA excitation and detection platform, enabled highly sensitive detection of two bacterial species. Validation experiments demonstrated that the sensor could clearly discriminate between the two species and exhibited high sensitivity across a broad concentration range (101–108CFU/mL), achieving limits of detection below 4 CFU/mL. The optical signal detection process was completed within one minute and exhibited excellent selectivity and stability.