Cytokine Milieu Relevant to Atopic Dermatitis Enhances Epidermal Trypsin‐Like Serine Protease Activity, Which Is Pharmacologically Suppressed in Human Keratinocytes
Abstract
Cytokine-driven enhancement and AD therapeutic suppression of epidermal trypsin-like serine protease activity in NHEKs.(A-F) NHEKs were stimulated with IL-4, IL-13, IL-17A, IL-22, IL-31, IFN-γ (50 ng/mL each), DEX, FK, DEL, T-5224, and/or DIF in the presence of 2 mM Ca 2+ for 96 h.Trypsin-like serine protease activity in the cultured media was measured using Boc-Phe-Ser-Arg-MCA as a specific substrate.*p < 0.05, **p < 0.01, ***p < 0.001.Data are mean ± SEM of triplicate samples and are representative of three independent experiments.AU, arbitrary unit; DEX, dexamethasone; FK, FK506; DEL, delgocitinib; DIF, difamilast.
Fields of Study & Subjects
Dermatology and Skin Diseases
Asthma and respiratory diseases
Contact Dermatitis and Allergies
Publication details
- Journal / source
- The Journal of Dermatology
- Volume
- 53
- Issue
- 3
- Pages
- 532–534
- Publication date
- 27 January 2026
- Language
- EN