Comparison of Taurine Extraction From Mytilus coruscus and Crassostrea gigas Using Ultrasound‐Assisted Enzymatic Hydrolysis

Ultrasound-assisted papain extraction of taurine from Mytilus coruscus and Crassostrea gigas was investigated in this study. Single-factor experiments and response surface optimization (RSM) were used to study the effects of three factors: enzyme addition amount, ultrasound time, and enzymatic hydrolysis time. Taurine content was detected using high-performance liquid chromatography (HPLC) to develop a species-specific taurine extraction process. According to response surface analysis, the optimal parameters for taurine extraction in M. coruscus were 2263.02 U/g of enzyme addition, 44.80 min of ultrasound time, and 4.55 h of enzymatic hydrolysis time, yielding a taurine extraction content of 4.10 ± 0.08 mg/g. The optimal parameters for taurine extraction from C. gigas were enzyme addition of 2738.05 U/g, ultrasound time of 35.84 min, and enzymatic hydrolysis time of 4.57 h. The taurine content was 3.27 ± 0.07 mg/g. Compared with boiling, enzymatic hydrolysis, ultrasonication, and 50°C water bath constant temperature methods, ultrasonic-assisted enzymatic hydrolysis represents increases of 77.45%, 25.08%, 56.49%, and 91.59% in taurine extraction content of M. coruscus and 185.42%, 41.35%, 116.56%, and 202.78% in taurine extraction content of C. gigas, respectively, indicating that the method has obvious advantages in the extraction of taurine. PRACTICAL APPLICATIONS: This study precisely optimized the taurine extraction process for Mytilus coruscus and Crassostrea gigas through ultrasonic-assisted enzymatic hydrolysis, providing species-specific solutions for the high-value processing of marine shellfish. By determining the optimal parameters for enzyme dosage, ultrasonication duration, and enzymatic digestion time for both species, this method significantly enhanced taurine extraction efficiency. This enables shellfish processing enterprises to customize production based on raw material characteristics, maximize resource utilization, and provide technical support for the sustainable development of shellfish aquaculture in coastal areas.